A comparative analysis of ADAR mutant mice reveals site-specific regulation of RNA editing

Complete absence of RNA editing in Adar1 KI Adar2 KO mice. (A) The body weights of wild-type (WT; n = 4 mice), Adar1^E861A/E861A Ifih^−/− mice (Adar1 knock-in [KI]; n = 6 mice), Adar2^−/−Gria2^R/R (Adar2 knockout [KO]; n = 9 mice), and Adar1 KI Adar2 KO (n = 11 mice) mice at 10 d of age are displayed as the mean ± SEM (Student's t-test, [**] P < 0.01, n.s., not significant). (B) Editing ratios of all sites examined in the cerebral cortex were compared between WT and Adar1 KI Adar2 KO mice. Values are displayed as the mean of the values of three WT and two Adar1 KI Adar2 KO mice. The red squares, blue circles, green diamonds, and gray triangles represent editing sites in coding sequences (CDS), microRNAs (miRNAs), repetitive elements (REs), and introns, respectively.