The inclusion of an alternative exon in RPS24 is significantly weakly correlated with hypoxia in human prostate tumors. (A) An alternative splicing event in RPS24 produces three amplicons in human prostate normal and tumor cDNA samples: short (S), long (L), and extra-long (XL). Because the extra-long amplicon was more abundant than in the cell lines (Supplemental Fig. S5), two PSI were calculated (L/S + L and XL/L + XL) each reflecting the inclusion of a single exon. Each PSI was correlated with the Ct (cycles to threshold in PCR) value of the hypoxia marker CAIX. The Spearman's correlation coefficient (r) and associated P-value is shown for 94 samples: normal tissue (n = 16), stage II (n = 40), stage III (n = 29), stage IV (n = 4), cancer stage not reported (n = 5). (B) Box plots compare PSI with tumor stage using one-way ANOVA (Kruskal–Wallis) with Dunn's multiple comparisons test. (+) represents mean. (*) P < 0.05, (**) P < 0.01. See Supplemental Material source data file for Figure 7.
