Hypoxia influences polysome distribution of human ribosomal protein S12 and alternative splicing of ribosomal protein mRNAs

The long variant of RPS24 is significantly up-regulated in spheroids of four cell lines. (A) Spheroids from four cell lines were imaged and harvested 5 d following cell seeding. (B) RT-qPCR using variant-specific RPS24 primers were used to confirm the ASPCR data from Figure 4. The ΔΔC_t method was used, normalizing to reference genes RPLP0 and RPL13A, and the fold-change made relative to normoxia. Data (n = 8), mean fold-change ± SEM. One-way ANOVA was performed on the ΔC_t values. (*) P < 0.05, (**) P < 0.01, (***) P < 0.001, (****) P < 0.0001. Scale bar, 500 µm.