Translational changes during oocyte maturation. (A) Extracts from oocytes at different stages of maturation were separated on sucrose gradients and the fraction of mRNA associated with polysomes was calculated. PCA analysis and plot shows the relationship between different oocyte stages and biological replicates. (B–E) Scatterplots comparing changes in mRNA polysome fraction between replicate extracts. Spearman correlation coefficients are indicated on the plots. (F) Histogram plot showing the fraction of mRNA associated with polysomes at different stages of oocyte maturation. (G) STEM cluster of translationally repressed mRNAs and associated GO terms. (H) STEM cluster of translationally activated mRNAs and associated GO terms. (I) STEM cluster of slowly translationally activated mRNAs and associated GO terms. (J) Western blots of candidate proteins predicted to be translationally activated during oocyte maturation. (K) Quantitation of western blots from two biological replicates.
