SYNCRIP is capable of regulating a set of miRNAs. (A,C) Real-time PCR analysis of pri-let-7a-1 and let-7a-5p in response to SYNCRIP knockdown (A) or overexpression (C), respectively. (B,D) Northern blot analysis of let-7a-5p miRNA in response to SYNCRIP knockdown (B) or overexpression (D), respectively. (E) Microarray expression profile of human miRNAs (fold change >1.5) in SYNCRIP knockdown samples (shSYNCRIP) compared with control sample (shctrl). Those miRNAs with adjusted P-value <0.05 were labeled in red. (F,G) Real-time PCR validation of candidate pri-miRNA (F) and mature miRNA (G) in SYNCRIP knockdown samples compared with control samples. (H,I) Northern blot analysis of miR-7-5p (H) and miR-1307-5p (I) in response to SYNCRIP knockdown (left panel) and overexpression (right panel). (J,K) Real-time PCR analysis of the expression of HMGA2 and EGFR, which are the target genes of let-7a-5p miRNA and miR-7-5p, respectively, in response to SYNCRIP knockdown (J) or overexpression (K). Significance in all real-time PCR analysis was determined by two-tailed t-test with (*) P < 0.05; (**) P < 0.01; (***) P < 0.001; ns, non-significant
