Forced isoform switching of Neat1_1 to Neat1_2 leads to the loss of Neat1_1 and the hyperformation of paraspeckles but does not affect the development and growth of mice

The expression patterns of marker genes were not significantly altered in the intestine and salivary gland of Neat1^ΔPAS/ΔPAS mice. (A) Schematics of the expression patterns of marker genes expressed in the intestinal epithelium. Note that the zonation of the enterocytes can be distinguished by different combinations of the marker genes. (B) In situ hybridization analyses of marker genes in the intestine. (C) Schematics of the expression pattern of the acinar cell marker Nkcc1 and the granular duct cell marker Ngf. (D) In situ hybridization analyses of marker genes in the salivary gland. Scale bars, 100 µm.