A convenient strategy to clone small RNA and mRNA for high-throughput sequencing

Quantification using a native PAGE gel. (A) PCR reactions produced perfectly based-paired normal (dotted box) products at low cycle numbers (12 and 15) and then bulged PCR products (dotted box) when the reactions were overcycled (18, 21, and 24). (B) Quantification using a PAGE gel: 5 µL PCR product was resolved using an 8% native PAGE gel. Dot-boxes are the small RNA amplicons for samples 1–7; M, the size marker.