A convenient strategy to clone small RNA and mRNA for high-throughput sequencing

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FIGURE 4.
FIGURE 4.

Cloning of ppp-RNA and csRNA. (A) The comparison of small RNAs cloned with PIR-1 or no PIR-1, as normalized to the total miRNA, which is equally cloned in both methods. (B) A capped RNA substrate is self-ligated (RNA–RNA) or circularized (a single RNA alone) by T4 RNA ligase 1 with hDcp2. (C) Cloned csRNAs within the pap-1 promoter region with “pink” representing sense reads and “blue” representing anti-sense reads.

This Article

  1. Published in Advance November 21, 2019, doi: 10.1261/rna.071605.119 RNA 26: 218-227

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