Deep sequencing of tRNA's 3′-termini sheds light on CCA-tail integrity and maturation

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FIGURE 4.
FIGURE 4.

1 nt-trailers are processed by RNase T with preference for adenosine. (A) Fractions of 1 nt-trailer precursors grouped by nucleotide identity show preference for adenosine over uridine. Points represent individual 1 nt-precursor fractions normalized by respective mature tRNA (n = 2), Wilcoxon rank-sum test. (B) Knockout of RNase T significantly increases the fraction of A-starting trailers and decreases U-starting trailers. Points represent individual 1 nt-precursor fractions normalized by respective mature tRNA (n = 2), bars represent means among all tRNAs within one group, Wilcoxon rank-sum test.

This Article

  1. Published in Advance November 12, 2019, doi: 10.1261/rna.072330.119 RNA 26: 199-208

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