FIGURE 2.
CCA-tail integrity varies among tRNA species and increases upon transition from exponential to stationary growth phase. (A,B) Fractions of intact (A) and nonintact (B) tRNAs measured by deep sequencing. Points represent mean fractions of individual tRNAs (n = 3), bars represent means among all tRNAs, Wilcoxon rank-sum test. (C,D) Intact (C) and nonintact (D) tRNA identified by ligation to specific oligonucleotides. Ligation was assessed by PAGE, SYBR Gold staining or Cy3 fluorescence detection.
