SOX9 facilitates the transcription of linc02095. (A) Promoter sequence of linc02095, showing potential consensus binding sites of SOX9 (highlighted in blue), SOX6 (highlighted in green), RUNX1 (highlighted in red) and 5′ end of transcript (highlighted in yellow). (B) Genomic position of SOX9 binding sites of linc02095 promoter. (C) Consensus SOX9 binding motifs, based on previous literature (Shi et al. 2015). (D) QPCR followed by SOX9 ChIP in M1 and M3 cells. Please note the increased association of SOX9 in the promoter region of linc02095 and S100P in M3 cells. S100P and CCNB1 were used as positive controls. The noncoding region from chromosome 17 was used as a negative control showing no binding of SOX9. SOX9 showed binding to linc02095 promoter in M3 cells as opposed to M1 cells. Data (D) represent mean ± SD of three independent experiments. P-value, (*) P < 0.05, was obtained by using Student's t-test.
