SOX9 facilitates the transcription of linc02095. (A) RNA polymerase II chip showing reduced association of RNA polymerase II at the promoter and gene body of SOX9 in linc02095-depleted M3 cells. B-actin and Ch17NC are used as positive and negative controls, respectively. (B) H3K4me3 chip showing no significant change in its association to the SOX9 promoter in control and linc02095 depleted cells. (C) RT-qPCR to quantify the stability of SOX9 mRNA in control and linc02095 depleted M3 cells treated with Flavopiridol (1M) for indicated time points (0, 1, 2, 3 h). (D) Nuclear and cytoplasmic fractionation of control and linc02095 depleted cells showing increased nuclear retention of SOX9 transcript in the absence of linc02095. Error bars in A–D represent ±SD of three independent experiments, that is, biological replicates. P-values, (*) P < 0.05, (**) P < 0.01, were obtained by using Student's t-test.
