Linc02095 and SOX9 promoted cell proliferation in BC cells. (A,i,ii). Plastic colony assay showing reduced colony numbers in cells depleted of linc02095 and SOX9. (B,a,b) Quantification of the data presented in A. (C,i) RT-qPCR showing relative levels of linc02095 in cells overexpressing linc02095. (C,ii) Plastic colony assay showing increased cell proliferation upon linc02095 overexpression. (C,iii) Quantification of data presented in Cb. (C,iv) RT-qPCR showing increased SOX9 mRNA levels in linc02095 overexpressed cells. (D,a,b) Anchorage independent growth assay showing reduced number of colonies in linc02095 and SOX9 depleted cells. (E) RT-qPCR to show relative expression of several cell cycle genes in linc02095-depleted cells. Error bars in B (i,ii), C (i,iii,iv), D (i,ii), and E represent ±SD of three independent experiments, that is, biological replicates. P-values, (*) P < 0.05, (**) P < 0.01, were obtained by using Student's t-test.
