Nuclear-enriched and polyadenylated linc02095 show elevated expression in BC cells. (A) RT-qPCR showing relatively high levels of linc02095 in M3, as compared to M1 and M4 cells. (B) RT-qPCR in nuclear and cytoplasmic fractionated cell extracts showing relative localization of linc02095, MALAT1, and actin RNA. MALAT1 (nuclear) and Actin (cytoplasm) are used as controls. (C) RT-qPCR analyses in poly(A)+ and poly(A)− fractionated M3 cells. 18SrRNA [poly(A)−] and actin [poly(A)+] were used as controls. (D) RT-qPCR showing relative levels of SOX9 mRNA in M1 and M3 cells. Error bars in A–D represent ±SD of three independent experiments, that is, biological replicates. P-values, (*) P < 0.05, (**) P < 0.01, were obtained by using Student's t-test.
