Interactions between SAM and the 5′ UTR mRNA of the sam1 gene regulate translation in S. pombe

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FIGURE 5.
FIGURE 5.

OsO4 probing of the tertiary structure of sam1 RNA on SAM binding. (A) Electropherogram of OsO4-modified RNA in the presence of 400 µM SAM (blue trace), no SAM (red trace), or blank control (gray trace). (B) SAM binding measured through changes in OsO4 reactivity at the 140U position in the RNA, on titration with SAM (data taken from Supplemental Fig. S3).

This Article

  1. Published in Advance November 25, 2019, doi: 10.1261/rna.072983.119 RNA 26: 150-161

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