Interactions between SAM and the 5′ UTR mRNA of the sam1 gene regulate translation in S. pombe

SAM binds to the sam1 UTR RNA. (A) Chemical structures of various compounds used to probe the binding characteristics of the sam1 UTR RNA. (B) The change in fluorescence intensity on titration of SAM with the fluorescein-labeled sam1 UTR RNA, the SAM binding curve is shown in the inset. Error bars are the standard deviations of at least three independent experiments. (C) The fluorescence intensity of the fluorescein-labeled sam1 UTR RNA–SAM complex samples from B under denaturing conditions. (D) SAH does not bind to the sam1 UTR RNA; fluorescence intensity measurements on titration of SAH with the fluorescein-labeled RNA.