FIGURE 2.
Methionine control of reporter gene expression through the 5′ UTR of sam1. (A) The Pnmt81-leaderRNAsam1/Met/cys-lacZα constructs used in this study. (B) β-galactosidase expression in S. pombe cells transformed with the UTR constructs as indicated. Cells were grown in EMM media in methionine 0 or 200 mg/L. Error bars are standard deviations of at least three independent experiments. (C) Real-time PCR analysis of mRNA abundance of the sam1 UTR RNA relative to the internal control tubulin.
