Interactions between the 5′ UTR mRNA of the spe2 gene and spermidine regulate translation in S. pombe

OsO₄ probing of tertiary structure of spe2 RNA on polyamine titration. (A) Analysis of OsO₄ modification electropherogram of RNA with 30 µM Spd (blue trace), compared with 0 µM Spd (red trace). (B) Histogram of OsO₄ modification of the in vitro transcribed RNA nucleotides (58–62) on titration of Spd. The reactivity of the nucleotide is calculated as the ratio of the height of the nucleotide to the height of all nucleotides. (C) Fitting curve for the ratio of OsO₄ modification signal of position U59 to that of full length on titration of Spd; KD is shown.