AS-NMD events are common in transcripts related to innate immunity. (A) Sashimi plots for an AS-NMD event identified in Mx1 from BMDMs stimulated with poly(I:C) for 0, 1, 4, 8, and 12 h. Sequenced RNA was derived from the total nuclear fraction. The y-axis represents reads per kilobase of transcript, per million mapped reads (RPKM). (B) Same as A for IKKε. (C) Same as A for Oasl2. (D) RT-PCR of Mx1 upon stimulation with poly(I:C) for 4, 8, and 12 h. (E) Same as D for IKKε. (F) Same as D for Oasl2. (G) GO terms enriched for AS-NMD events, as compared to a background of expressed genes. (H) Schematic representation of major pathways in the viral pathogen response. Red arrows are shown above factors containing AS-NMD events. Data are representative of two independent experiments (D–F). Genomic coordinates represent the mm9 genome assembly.
