Human Oas1 is regulated at the posttranscriptional level through productive and unproductive AS. (A) Depiction of the mRNA splice isoforms found in human Oas1. There exists a single G/A SNP in the OAS1 exon 6 splice-acceptor (rs10774671), with the G variant producing the more active p46 isoform. (B) Sashimi plots depicting the productive AS event at the 3′ end of human Oas1. RNA is from PBMCs stimulated with IFN-α derived from two healthy donors. Genomic coordinates represent the hg19 genome assembly. (C–E) Sashimi plots for an AS-NMD event identified in exon 3 of human Oas1 from healthy donor derived PBMCs either unstimulated (top) or stimulated with IFN-α (C), A549 cells uninfected (top) or infected with influenza virus PR8 (D), or primary macrophages from two separate donors infected with West Nile virus (E). Genomic coordinates represent the hg19 genome assembly. All sequencing samples in Figure 3 are derived from whole-cell RNA.
