Ribosome-wide profiling of 2′-O-Me in zebrafish developmental stages and adult fish. (A) Graph depicting fraction methylated at all methylated sites in five developmental stages. Sites (x-axis) are numbered according to human rRNA to allow comparisons and novel sites found in this study are highlighted in bold. The corresponding nucleotide positions for zebrafish early- and late-rRNA can be found in Supplemental Table S2. Data from different stages are indicated by color and asterisks indicate the levels of statistical significance when comparing the sample in question to all other samples. Error bars indicate the standard deviation, n = 3. (B) Examples of differentially methylated sites between the five stages analyzed. Zebrafish novel methylations are highlighted in bold. Asterisks and error bars were used in the same way as in A, although coloring was omitted.
