RNA-seq accuracy and reproducibility for the mapping and quantification of influenza defective viral genomes

DVG landscapes for the WT and transcription-deficient A/WSN/33 viruses. The DVGs identified upon DG-seq analysis of vRNAs purified from the WT, PA-K635A and PA-R638A viral stocks in Expt 1 were characterized separately for each virus (while they were analyzed all together in Fig. 2C). (A) The total DVG frequency is indicated for each genomic segment. (B) 2D plots indicate, for each identified DVG represented by a dot, the breakpoint start and end positions and the frequency (size of the dot). The axes scale is adapted according to the size of each segment.