FIGURE 1.
Detection and quantification of DVGs using DG-seq. (A) Schematic representation of the DG-seq pipeline. (B) DG-seq can detect deletion DVGs. (C) Distribution of the number of reads supporting a given deletion (with unique breakpoint start and end) upon analysis of in vitro transcribed full-length pseudo-vRNAs. For instance, in RT-seq samples most deletions (>650) are covered by one read, about 100 are covered by two reads, etc. The 99th percentile was found identical in RT-seq and RT-PCR-seq samples. (D) Some DVG junctions cannot be identified because of too short sequences on one side of the junction. (E) Typical coverage per site depending on DVG frequency.
