Total RNA edits in A6 associated with RESC6-IPs upon KH2F1–RNAi: (A) Site-by-site, and (B) cumulative analysis A6 mRNA. T-str (71–115) and ES positions, including ES1 and the stop codon (square) are indicated. Positions upstream of T-str 71 are omitted because the small number of edits at those sites have a negligible effect on the cumulative plot. The gRNAs (blue bars) mostly derive from an extensive gRNA transcriptome study (Koslowsky et al. 2013). The edited 3′ UTR region anneals with gRNA-1 and gRNA-2. We used an alternative gRNA-1 transcript. Variants of gRNA-1 with identical guiding capacity were sequenced in strains EATRO 164 and Lister (Koslowsky et al. 2013; Madina et al. 2014) or predicted in sequenced minicircles in strain EATRO 1125 (Cooper et al. 2019). gRNA-2 was predicted in strain EATRO1125. Values and error bars reflect mean of n = 2 +SD independent biological replicates. Sliding window analyses are included comparing −Tet to +Tet day 4 (top tier) or +Tet day 3 (bottom tier).
