RNA editing accuracy in mtRNA, KREH2-IPs, and RESC6-IPs: (A) Site-by-site, and (B) cumulative Inc/Cor analyses in the RPS12 ORF sequence. Inc/Cor values are scored as the percentage of RNA-seq reads with incorrect editing divided by the percentage of RNA-seq reads with correct editing, according to the canonical fully edited RPS12 sequence. For clarity in B, RPS12 is divided into two regions (T-str positions 31–88 and 89–151, respectively), each with a separate plot of cumulative Inc/Cor. Major differences in Inc/Cor between samples include those at T-str positions 48 and 80 (ES57 and ES46, respectively; filled arrowheads). (C) Distribution of high-frequency incorrect edits (Inc) in RESC6-IPs. Color-coded arrowheads indicate high-frequency reads with incorrect editing (>5% to >30%) at those positions. The RPS12 3′ half included most high-frequency reads of incorrect editing (horizontal broken line). Blue bars represent frequent gRNAs for RPS12 as in Figure 2. Values and error bars reflect mean of n = 2 +SD independent biological replicates. Sliding window analyses are included as in Figure 2 comparing RESC6-IP to mtRNA (top tier) or KREH2-IP to mtRNA (bottom tier).
