“Snapshot” of a typical data set collected using targeted RNA-seq analysis of RPS12 amplicons in this study. Stacked histogram of editing events at each position in RPS12 in a representative replicate sample of RESC6-IP. The cDNA fragment examined is shown as a reference T-stripped sequence (T-str) spanning the internucleotide positions T-str 17 to 151 (see the canonical fully edited RPS12 ORF and 5′ UTR “B-form” sequence in Supplemental Fig. S1). A canonical editing site (ES) for uridine insertion (Ins) or deletion (Del) is just 5′ to a red or a blue non-T nucleotide, respectively. A noncanonical editing site (nES) is just 5′ to a black non-T nucleotide. The representative histogram shows the percentage of RNA-seq reads at each position: ESs with correct insertion (red) or deletion (blue), and incorrect “partial” editing events (yellow). The remaining reads contain a preedited sequence. Editing events at nESs were scored (black). nESs do not require sequence changes in mature transcripts. However, all nESs at steady state carried editing events in our samples. The start codon (box) includes T-str positions 30–31 and requires editing at T-str 31 (ES72). The stop codon (box) includes T-st 151 (ES3) and requires editing at this position.
