In vitro studies provide insight into effects of Dicer-2 helicase mutations in Drosophila melanogaster

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FIGURE 7.
FIGURE 7.

Effects of helicase mutations on ATP hydrolysis. (A) PhosphorImages of representative TLC plates show hydrolysis of α-32P-ATP incubated with Dcr-2WT, Dcr-2F225G, or Dcr-2G31R, with BLT (left) or 3′ovr (right) 52-dsRNA, o, origin. (B) Quantification of data from panel A (mean ± SD, n = 3) fit with the pseudo first-order equation as in 4B.

This Article

  1. Published in Advance August 25, 2020, doi: 10.1261/rna.077289.120 RNA 26: 1847-1861

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