Helicase mutations affect processing of viral dsRNA and viral load. (A) Representative data for siRNA reads from one biological replicate mapping to DCV, three DPI (left), and VSV, five DPI (right), normalized to number of miRNA reads (see Supplemental Fig. S4A for additional replicate). (PFU) plaque forming unit. Genome and antigenome reads are colored gray and dark brown, respectively. (B) Comparison of Dcr-2 processing efficiency using the ratio of 21 nts to other product reads (17–30 nts in length) for DCV (top) and VSV (bottom), three and five DPI, respectively. Analysis was done for reads of the genome and antigenome, and data normalized to highest GFP::Dcr-2WT antigenome ratio. When multiple data sets exist, n = 2, error bars, SD. (C) Viral load was measured by RT-qPCR for DCV (top) and VSV (bottom), at various days post infection. Normalization was to the housekeeping gene RP49, and data were plotted relative to the earliest time point. n = 3–4, with at least two biological replicates, with six males and six females in each replicate; error bars, SD.
