Mutations in the helicase domain have different effects on processing of a transgene encoding an inverted repeat hairpin. (A) Eye color of indicated genotypes (see Materials and Methods) is shown on the left panel for representative male and female flies, with absorbance at 485 nm plotted to the right. Mean shows average of three independent experiments with biological replicates, with each replicate containing 10 female heads; error bars, SD. Each sample is compared to the parental line CantonS. Kruskal–Wallis test. (*) P < 0.05; (**) P < 0.01; (***) P < 0.001; (ns) not significant. (B) Size distribution of GMR-wIR mapped reads, normalized to miRNA reads. Replica data sets (libraries) are color coded according to strain, with the right panel showing a magnification of a section of the left plot. Six libraries were analyzed for dcr-2null and GFP::Dcr-2F225G, and five libraries for other genotypes. (C) Representative plots of wIR coverage of 21 nt long siRNAs for indicated strains normalized to the number of miRNAs in each library. Reads were mapped to hairpin sequence and data color coded as in cartoon.
