Alu siRNAs induce apoptosis. (A) Fluorescence microscopic detection of apoptosis induced in Alu siRNA-transfected HeLa cells in comparison to the cells treated with a known inducer of apoptosis, Staurosporine (2 µM, 2 h). Apoptosis was evaluated with the CellEvent Caspase-3/7 Green apoptosis-detection system (Life Technologies). (B) Apoptosis induced in CDCP1 knockdown HeLa cells was also evaluated using the CellEvent Caspase-3/7 Green apoptosis-detection system. Scale bars, 200 µm. (C) The extent of apoptosis induced by Alu siRNA transfection or CDCP1 knockdown was analyzed by western blotting of cleaved poly(ADP-ribose) polymerase (PARP). CDCP1 bands represent the full-length protein (135 kDa), whereas PARP bands represent the cleaved protein (89 kDa).
