Reduced expression of Alu siRNAs in ADAR1 or DICER depleted cells. (A) The length distribution of read numbers of sense and antisense Alu siRNAs reduced by ADAR1 knockdown in HeLa cells. (B) The length distribution of read numbers of sense and antisense Alu siRNAs reduced by DICER knockout in HEK293T cells. Read numbers were first normalized: The read number of Alu siRNAs was divided by the read number excluding miRNA and Alu siRNAs from the total small RNA read number and multiplied by one million. Then, the normalized Alu siRNA read number of ADAR1 knockdown (siADAR1 HeLa) or DICER knockout (DICER null HEK293T) cells was subtracted from that of control cells (siControl HeLa or WT HEK293T), and the difference was plotted on the y-axis. A negative number on the y-axis indicates a decrease in the normalized number of reads after ADAR1 knockdown or DICER knockout. The x-axis is the number of bases in the siRNA sequence.
