FIGURE 3.
Data-guided RNA secondary structure prediction of medaka and human CR4/5 domain. SHAPE (1M7) reactivity data were used as weights to guide RNA structure prediction for medaka (A) and human (B) CR4/5 domains. Using the Biers package of HiTRACE, RNAstructure models of each RNA domain were calculated with 100 bootstrap replicates, while varying the SHAPE slope parameter in intervals of 0.2 kcal/mol. The abundance of each helical RNA element (Confidence) derives from the bootstrap replicates and is plotted for each respective value of SHAPE slope. Ref. Pin refers to Reference hairpin.
