pTIN/pTIN7m plasmid map. Features of the pTIN/pTIN7m vector are depicted. The positions of the indicated restriction sites are shown. The DNA sequences of the WT or mutant (mut) Pho7 DNA binding site present in the pTIN or pTIN7m vector, respectively, and the base-specific changes for mutations in the DSR element cluster are indicated on the top right. The sequences of the following regions are shown at the bottom right—the nmt1 promoter fused to the nc-tgp1 lncRNA transcription start site (TSS), and the tgp1 5′ UTR with the multiple cloning site polylinker (MCS), where the black bent arrow marks the initiating nucleotide of the lncRNA or the mRNA as indicated.
