The Ribo-Pop probe design pipeline can be applied to other organisms. (A) The Ribo-Pop design pipeline. After software installation, the remaining steps can be automatically deployed. (B) An example output figure of the Ribo-Pop design pipeline after running the pipeline on the major rRNA transcripts of S. cerevisiae and S. pombe yeasts. The probe selection process for the large rRNA transcript is shown. Gray dots indicate possible probe placement sites based on conservation and sequence composition. Blue dots indicate the selected probes sites based on a desired probe set size of 10. (C) The Ribo-Pop probe set designed to target S. cerevisiae and S. pombe was applied to 100 ng of total RNA from S. cerevisiae. The averages of three experiments using different RNA samples are shown. Error bars are standard deviation.
