FIGURE 3.
Depletion of rRNA performs well across a range of total RNA input levels. The percent of 18S, 28S-L (28L), and 28S-R (28R) targets remaining after Ribo-Pop starting from various levels of input RNA are shown. qPCR values for each target were normalized to the Act5c level of the same sample and then normalized to an input sample that was not subjected to Ribo-Pop treatment. See Materials and Methods and Supplemental Protocol for a detailed protocol. Error bars are the standard deviation between three independent experiments, each using a different RNA sample. Sequencing data from the 5 µg samples are presented in Figure 4.
