FIGURE 3.
Reproducibility and sensitivity of pri-miRNA processing efficiency determination. (A) Correlation of processing efficiency determined from high-depth sequencing of chromatin-associated RNA (Conrad et al. 2014) and low-depth sequencing of targeted sequencing of total RNA for two technical replicates. (B) Sensitivity of each approach determined by RPKM at pri-miRNAs. Slope shows a 6670-fold increase in sensitivity and a corresponding decreased need for sequencing depth. RPKM for targeted sequencing is calculated as the average of two independent enrichment replicates.
