microRNA-seq of cartilage reveals an overabundance of miR-140-3p which contains functional isomiRs

Evidence for miR-140-3p.1 and miR-140-3p.2 function during human MSC chondrogenesis. Re-analysis of data from Barter et al. for human MSC differentiation to cartilage. (A) Changes in gene expression for eight MSC and chondrogenic markers. (B) Mean fold change of all genes that are predicted targets of either a miR-140-5p (green), miR-140-3p.1 (red), or miR-140-3p.2 (blue) during MSC chondrogenesis. (C) Sylamer analysis for a miR-140-5p (green), miR-140-3p.1 (red), and miR-140-3p.2 (blue) 7m8 seed sequences.