
Presence of unusual pairs in AGC-tRNAAla isodecoders in various branches of the phylogenetic tree. We checked the tRNA predictions present in GtRNAdb (Chan and Lowe 2016). As there were too many sequences, we used a threshold for the tRNAscan-SE score of 60. For the Spermatophyta, this represents 242 sequences and for the Vertebrata 199. The proportions of AGC-tRNAAla isodecoders vary strongly depending on species. Standard base pairs are in black and unusual pairs in red. The tree was built by the Taxonomy Common Tree tool (https://www.ncbi.nlm.nih.gov/Taxonomy/CommonTree/wwwcmt.cgi) and drawn using the software FigTree v1.4.4 (https://github.com/rambaut/figtree/).










