FIGURE 3.
Interactions between the P-site tRNA and the large rRNA in bacterial ribosome (A,B) (from Watson et al. 2020) and in a eukaryotic ribosome (C,D) (from PDB 6AZ1, 6AZ3; Shalev-Benami et al. 2017). The contacts occur in the minor groove of base pairs 29–41 and 30–40 in the anticodon stem. Notice how the hydroxyl groups of 40 and 41 are forming H-bonds locking in those residues. Further, A1338 (A1996 in the eukaryotic ribosome) formed a trans-Hoogsteen/Watson–Crick pair (a “sheared” base pair) with G944 (G1521, respectively). Drawings made using PyMOL (PyMOL 1.7.7.6—Incentive Product © Schrodinger LLC).
