Inefficient splicing of PRP5 orthologs in the Saccharomycotina and A. fumigatus disrupts the ORF and likely serves to feedback inhibit PRP5 expression. (A) Splicing pattern is shown for each of the species analyzed, along with the species phylogeny as in Figure 1A. N.D. indicates that splicing pattern could not be determined because of the low expression level in the conditions analyzed. (B) Splicing of the first intron of the PRP5 ortholog in A. fumigatus disrupts the ORF by removing the start codon. (C) The inefficiently spliced intron has been lost from the PRP5 ortholog in L. starkeyi. (D) Splicing of the first intron of the PRP5 ortholog in C. jadinii disrupts the ORF by removing the start codon. (E) Splicing of the first intron of PRP5 in S. cerevisiae disrupts the ORF by removing the start codon, as previously reported. In panels B–D, only the 5′ end of the gene is shown.
