Origin, conservation, and loss of alternative splicing events that diversify the proteome in Saccharomycotina budding yeasts

Alternative splicing of FES1 orthologs in the Saccharomycotina. (A) Splicing pattern is shown for each of the species analyzed, along with the species phylogeny as in Figure 1A. (B) Y. lipolytica diverged before the likely origin of the FES1 retained intron and no splicing was detected. (C) Intron retention in the FES1 ortholog from O. polymorpha. (D) Intron retention in the FES1 ortholog from K. lactis. (E) The retained intron has been lost from the FES1 ortholog in T. blattae. (F) Intron retention in the FES1 ortholog from S. cerevisiae as previously reported. NLS indicates the location of a nuclear localization signal.