Alternative splicing of SRC1/HEH2 orthologs in the Saccharomycotina and A. fumigatus. (A) Splicing pattern is shown for each of the species analyzed, along with the species phylogeny as in Figure 1A. The black asterisks indicate a change from alternative 3′ splice sites to alternative 5′ splice sites or vice versa between A. fumigatus and the Saccharomycotina, changes in the position of the minor 5′ splice site in the Candida genus and the Saccharomycetacea, and the addition of an intron retention variant in the ZT clade. (B) Alternative 3′ splice sites in the SRC1/HEH2 ortholog in A. fumigatus. (C) Alternative 5′ splice sites in the SRC1/HEH2 ortholog in Y. lipolytica. (D) Alternative 5′ splice sites in the SRC1/HEH2 ortholog in L. kluyveri. (E) Alternative 5′ splice sites and intron retention in the SRC1/HEH2 ortholog in Z. rouxii. (F,G) Duplicated SRC1 and HEH2 genes in S. cerevisiae. SRC1 uses alternative 5′ splice sites, as previously reported, while HEH2 lacks an intron.
