Alternative splicing of GCR1 orthologs in the Saccharomycetacea. (A) Splicing pattern is shown for each of the species analyzed, along with the species phylogeny as in Figure 1A. A clear ortholog of GCR1 is not present outside the Saccharomycetacea. (B) The K. lactis GCR1 ortholog does not contain an intron. (C) The C. glabrata GCR1 ortholog contains an intron, but no alternative 5′ or 3′ splice sites. Translation from an AUG codon in the intron the predicted to produce an alternate isoform. (D) Splicing of GCR1 in S. cerevisiae is inefficient and uses alternative 3′ splice sites, as previously reported. It may also use alternative 5′ splice sites at low frequency. Translation from an AUG codon in the intron produces an alternate isoform.
