Global discovery of bacterial RNA-binding proteins by RNase-sensitive gradient profiles reports a new FinO domain protein

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FIGURE 6.
FIGURE 6.

FopA protects Inc and anneals it to the 5′UTR of repZ. (A) Purified FopA with cleaved-off tag. (B) FopA bound Inc, repZ, and the Inc–repZ complex with an apparent KD about 1 µM. n = 2. (C) FopA accelerated Inc*–repZ complex formation. k2 represents the rate constant. n = 2. (D) FopA protected full-length Inc alone and in the complex with repZ from RNase A degradation at loop regions. n = 2.

This Article

  1. Published in Advance July 9, 2020, doi: 10.1261/rna.076992.120 RNA 26: 1448-1463

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