Global discovery of bacterial RNA-binding proteins by RNase-sensitive gradient profiles reports a new FinO domain protein

RBPs shift and reduce distribution in the gradient. In addition to a shift to LMW fractions, the presence and distribution of Hfq, ProQ, CspD, and CsrA were reduced in the gradient upon RNase treatment (quadrant I). Ribosomal proteins increased distribution in the gradient while still shifting to LMW fractions (II). Some proteins shifted to HMW fractions upon RNase treatment or pelleted (III, IV).