Effects of amino acids and yfmH translation on expression of yfmG. (A) Schematic representation of the PyfmHG-yfmH-lacZ transcriptional fusion (Fusion 1), PyfmHG-lacZ transcriptional fusion (Fusion 2), Ptrp-yfmH-lacZ transcriptional fusion (Fusion 3), PyfmHG-yfmH’-‘lacZ translational fusion (Fusion 4), and the PyfmHG-yfmHG’-‘lacZ translational fusion (Fusion 5). Transcription start site is indicated with an arrow, and the terminator is indicated with a stop sign. yfmH start to stop codon mutations are indicated by a red x. (B–D) β-galactosidase assays of the indicated chromosomally integrated fusions when cultures were grown in minimal + glutamine media (M), ACH + tryptophan (A), minimal + lysine and arginine (2), minimal + leucine, isoleucine and valine (3), or minimal + the thirteen amino acids encoded in yfmH (13). [*] Start-to-stop codon mutation in yfmH. All cultures contained glutamine. Each experiment was performed at least three times. Values are averages ± standard deviation.
