Amino acid responsiveness of the yfmG 5′UTR element. (A) Formylglycine generating enzymes catalyze the conversion of cysteine into formylglycine. (B,C), Secondary structures constrained with in vivo Structure-seq2 data in the absence (B), and presence (C) of amino acids. Notably, a known site of termination is shown as a red arrow with the terminator hairpin boxed (Mondal et al. 2016). (D) A CircleCompare plot showing changes in the RNA secondary structure from Structure-seq2 data upon amino acid starvation. Yellow lines are base pairs predicted only in the absence of amino acids, blue lines are base pairs predicted only in the presence of amino acids, and gray lines are shared base pairs.
