Structure-seq2 probing of RNA structure upon amino acid starvation reveals both known and novel RNA switches in Bacillus subtilis

Change in transcript DMS reactivity and change in abundance are inversely correlated overall and in the CDS, but not in the 5′UTR or 3′UTR. (A) Change (Δ) in transcript mean DMS reactivity (mean DMS reactivity_−AA − mean DMS reactivity_+AA) was plotted against change (Δ) in transcript abundance [log₂(TPM_−AA) − log₂(TPM_+AA)]. These transcripts were then broken down into their component regions and the analysis repeated; (B) 5′UTR, (C) CDS, and (D) 3′UTR. A table summarizing all relevant tests and statistics for this figure can be found in Supplemental Table T.3.