Pdc2/Pat1 increases the range of decay factors and RNA bound by the Lsm1–7 complex

Multiple RNA sequences drive higher order PatMC/Lsm1–7 assembly in a Pat1-dependent manner. (A) SEC-MALS of 20.6 µM PatMC/Lsm1–7 with stoichiometric U15 RNA. The expected molar mass of the monomeric PatMC/Lsm1–7 complex is 133 kDa. (B) Representative SDS-PAGE gel of fractions in A. (C) SEC-MALS of 20.6 µM Lsm1–7 with stoichiometric U15 RNA. The expected mass of the monomeric Lsm1–7 complex is 81 kDa. Expected and observed molar masses are shown in Table 1. The light scattering (blue) and molar mass (red) are displayed. (D) Stoichiometry analysis of Lsm1–7 or PatMC/Lsm1–7 with 5′-FAM labeled U15 RNA at 0.5 µM, which is >100-fold above K_d.