Pdc2/Pat1 increases the range of decay factors and RNA bound by the Lsm1–7 complex

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FIGURE 2.
FIGURE 2.

RNA promotes stable dimerization of the PatMC/Lsm1–7 complex. (A,B) SEC-MALS of 20.6 µM PatMC/Lsm1–7 with stoichiometric (A), A15 and (B), A10 RNA. Expected and observed molar masses are shown in Table 1. The light scattering (blue) and molar mass (red) are displayed. (C) SV-AUC of 9.3 µM PatMC/Lsm1–7 with stoichiometric A15 RNA at 250 mM NaCl. c(S) is the sedimentation distribution with molecular weights determined from fits.

This Article

  1. Published in Advance June 8, 2020, doi: 10.1261/rna.075812.120 RNA 26: 1380-1388

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